IJIMT 2014 Vol.5(2): 120-123 ISSN: 2010-0248
DOI: 10.7763/IJIMT.2014.V5.498

Microbial Community and Population Dynamics Analysis of Anaerobic Fermentation of Vegetable Waste under Different pH

Hailing Ma, Mingyue Zheng, Mingxia Zheng, Guochen Zhang, and Kaijun Wang
Abstract— The advantage bacterium group of acidogenic and methanogenic processes by PCR-denaturing gradient gel electrophoresis (DGGE) under different pH values(4.1~4.3 and 5.9~6.1) was studied . 16S rDNA of bacteria and methanogenic archaea amplified with two universal primers (341F-GC /109F-GC and 518R). PCR amplicons were separated by denaturing gradient gel electrophoresis (DGGE) using the DGGE Decode system. Parts of the separated DNA were sequenced after purification from DGGE gel. The sequences of several 16S rDNA DGGE fragments were determined and some possible bacteria were confirmed in comparison in GeneBank (NCBI).The results showed that the acidogenic phase of fruits and vegetables during the fermentation process display different type: ethanol-type and butyrate-type fermentation and the predominant bacterium act differently. The predominant organism groups of methanogenic processes within the reactor performed differently, too. These results indicated that the change in the product formation was mainly caused by the change of the dominant microbial populations under different pH conditions.

Index Terms— DGGE, different pH conditions, acidogenic phase, the predominant organism groups.

Hailing Ma was with China University of Geosciences, Beijing, China. she is now with the School of Environment, Tsinghua University, Beijing, China (e-mail: mahailing001@126.com).
Kaijun Wang is with the School of Environment, Tsinghua University, Beijing, China.

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Cite: Hailing Ma, Mingyue Zheng, Mingxia Zheng, Guochen Zhang, and Kaijun Wang, " Microbial Community and Population Dynamics Analysis of Anaerobic Fermentation of Vegetable Waste under Different pH," International Journal of Innovation, Management and Technology vol. 5, no. 2, pp. 120-123, 2014.

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